Figure 4. Distinct downstream effectors are important for tolerance of S. cerevisiae to different ergosterol biosynthesis inhibitors.

To dissect the role of downstream effectors of Slt2 in tolerance to ergosterol biosynthesis inhibitors, we tested the impact of their deletion individually and in combination on drug susceptibility in an MIC assay. Data was analyzed after 72 hours at 35°C in SD medium as in Figure 1A. The minimum drug concentration that inhibits growth by 80% relative to the drug-free growth control (MIC₈₀) is indicated for each strain.