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. 2010 Aug 26;6(8):e1001075. doi: 10.1371/journal.ppat.1001075

Figure 1. egl-9-mediated resistance to Pseudomonas aeruginosa PAO1 fast killing is mediated by hif-1.

Figure 1

A. Percent C. elegans survival over time on P. aeruginosa PAO1. Wild-type N2 and hif-1 mutants exhibit similar sensitivity to fast killing (>50% dead by 40 minutes), but 100% of animals carrying a strong loss-of-function mutation in egl-9 survived after 2 hours. The P. aeruginosa resistance phenotype conferred by egl-9(sa307) was suppressed by the hif-1(ia04) strong loss-of-function allele. B. Stabilization of HIF-1 protein was not sufficient to confer resistance to P. aeruginosa PAO1 fast killing. The survival curves for strains expressing epitope-tagged HIF-1 or stabilized HIF-1(P621G) were similar to that of the wild-type strain. Three independently generated transgenic strains expressing HIF-1(P621G) were assayed. Each experiment was repeated at least three times with 30–50 animals for each strain. The error bars represent standard errors. These data are also presented in tabular format in Text S1.