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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Nat Neurosci. 2010 Aug 15;13(9):1128–1136. doi: 10.1038/nn.2614

Figure 5. Mecp2 mutant mice show altered induction of Fos/Jun family IEGs.

Figure 5

a–d, c-Fos expression in the NAc of Mecp2+/y (WT) and Mecp2308/y (MUT) mice that received repeated Veh (Veh:AMPH) or AMPH (AMPH:AMPH) treatments and AMPH at challenge. Scale bar indicates 50μm. e, Quantitation of c-Fos expression as shown in panels a–d. ANOVA indicated a treatment by genotype effect (F3,26=4.36, p=0.048; * - p=0.006, Veh:AMPH-WT compared to AMPH:AMPH-WT; # - p=0.05, AMPH:AMPH-WT compared to AMPH:AMPH-MUT). c-Fos expression in MUT mice is not different between the Veh:AMPH and AMPH:AMPH conditions (p=0.90). f–i, FosB and JunB following acute AMPH challenge. Scale bar indicates 45 μm. j–k, Quantitations of FosB and JunB expression. j, In WT mice, FosB expression is reduced after repeated AMPH treatment (ANOVA; treatment by genotype: F3,23=11.01, p=0.003; * - p<0.001, Veh:AMPH-WT compared to AMPH:AMPH-WT). In MUT mice, the single injection of AMPH at challenge did not induce FosB expression as compared to WT animals ((x002C6) - p=0.001, Veh:AMPH-WT compared to Veh:AMPH-MUT), and there was no difference between the Veh:AMPH-MUT and AMPH:AMPH-MUT groups (p=0.96). k, Repeated injection of AMPH upregulates JunB expression in WT mice (ANOVA; treatment by genotype: F3,21 = 10.929, p=0.004; * - p=0.01, Veh:AMPH-WT compared to AMPH:AMPH-WT). For MUT animals acute AMPH injection at challenge caused JunB expression to be significantly elevated compared with similarly-treated WT mice (ˆ - p<0.001, Veh:AMPH-WT compared to Veh:AMPH-MUT); however, there was no statistical difference in JunB expression between the Veh:AMPH-MUT and AMPH:AMPH-MUT groups (p=0.47). Error bars indicate mean ± s.e.m.