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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Nat Neurosci. 2010 Aug 15;13(9):1128–1136. doi: 10.1038/nn.2614

Figure 8. Increases in pMeCP2 correlate with behavioral sensitization following repeated AMPH administration.

Figure 8

a, Experimental scheme. Arrows represent injections. n=9-10/group. b, Cumulative distance traveled over 1 h following challenge with Veh or AMPH. ANOVA revealed a significant main effect of treatment [F3,35=206.3, p<0.001]. * - ps<0.0001, Veh:Veh or AMPH:Veh compared to Veh:AMPH or AMPH:AMPH groups; # - p=0.025, Veh:AMPH compared to AMPH:AMPH. c-f, pMeCP2 immunofluorescence in the NAc 2 h after challenge injections of Veh (c,d) or AMPH (e,f) from mice that received repeated injections of Veh (c,e) or AMPH (d,f). Scale bar indicates 30 μm. g, Quantitation of pMeCP2 immunofluorescence intensity in the NAc. ANOVA indicated a significant effect of treatment (F3,27=8.3, p<0.001). * - p=0.011, Veh:Veh compared to Veh:AMPH, and p=0.017, Veh:Veh compared to AMPH:AMPH; # - p=0.007, AMPH:Veh compared to Veh:AMPH, and p=0.013, compared to AMPH:AMPH. h–k, Pearson product correlations of pMeCP2 immunofluorescence intensity in the NAc and cumulative locomotor activity from individual animals. h,i, pMeCP2 expression does not correlate with locomotor activity in mice that received Veh injection (Veh:Veh and AMPH:Veh) at challenge, r=0.17, p=0.26 (Veh:Veh) and r=0,55, p=0.096 (AMPH:Veh), n=9 and 10. j, pMeCP2 expression does not correlate with locomotor activity in mice that received a single AMPH injection (Veh:AMPH) at challenge, r= -0.073, p= 0.80, n=15. k, pMeCP2 expression strongly correlates with locomotor activity in animals that received repeated AMPH injections and AMPH at challenge (AMPH:AMPH), r=0.61, p=0.0087, n=17. Error bars indicate mean ± s.e.m.