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Histological examples of periosteum/PCL composites cultured in spinner flask bioreactors for six weeks. Sections of the engineered cartilage were stained with safranin O/fast green. (A & B) Minimal cartilage was produced from periosteum/PCL composites cultured under static conditions (0 rpm), as indicated by safranin O/fast green stain (red). Significantly more cartilage was produced under fluid flow, 20 rpm (C &D), 60 rpm (E & F), and 150 rpm (G&H).
