AHR and non-eosinophilic lung inflammation are not impaired by the absence of IL-4Rα-mediated signaling in the Th2 asthma model. Evaluations (n = 5 each group) were performed twice after allergen challenge. *, P < 0.05 compared to mice sensitized with PBS; **, P < 0.05 versus the other groups. (A) Levels of cytokines in the BAL fluids of the IL-4Rα-/- and WT (BALB/c) mice 6 h after three rounds of allergen challenge. (B) Methacholine AHR 24 h after the final allergen challenge. (C) BAL cellularity (left panel) and representative lung histology (right panel) in IL-4Rα-/- and WT mice 48 h after the final allergen challenge. (H&E staining, original magnification ×200). a, WT_PBS; b, IL-4Rα-/-_PBS; c, WT_OVA/alum; d, IL-4Rα-/-_OVA/alum. (D) Levels of TGF-β1 (upper panel) and IP-10 (lower panel) 48 h after the final allergen challenge.