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. 2010 Jul 19;42(8):583–595. doi: 10.3858/emm.2010.42.8.060

Figure 6.

Figure 6

Knockdown of Akt impairs NGF and BDNF mediated cell survival. (A) Scr RNA or si-Akt transfected cells were exposed to 100 nM STS in the presence or absence of NGF or BDNF. Genomic DNA was extracted and 10 µg of DNA was loaded on a 2% agarose gel. (B) Scr RNA or si-Akt transfected cells were stimulated with NGF or BDNF. After exposure to 100 nM STS, 30 µg of cell lysate was immunoprobed with anti-PARP antibody. The silencing effect of si-Akt was examined by anti-Akt antibody. (C and D) The H19-7 cells (left) and hippocampal neuron cells (right) were transfected and treated as described in (B). The nuclei were stained with DAPI and counted under a fluorescent microscope. *P < 0.05. (E) Genomic DNA was extracted from si-Akt trasnfected H19-7 cells and loaded on a 2% agarose gel (upper panel). 30 µg of cell lysate was immunoprobed with anti-PARP antibody. The knockdown effect and the loading control are shown by the anti-Akt and anti-β actin antibodies, respectively (lower panels).