Figure 7.

Inhibition of ICAM-1 and neutrophil depletion reduce anti-GBM antibody-induced glomerular platelet recruitment within glomeruli. A: To assess the role of ICAM-1 in glomerular platelet adhesion, mice were pretreated with a mAb against ICAM-1 (200 μg, i.v., n = 7) or isotype control mAb (n = 7) and anti-GBM antibody-induced platelet recruitment assessed as performed previously. Data are shown as mean ± SEM. *P < 0.05 versus isotype control group for each time point. B: To determine the effect of neutrophil depletion on glomerular platelet recruitment, mice were pretreated with RB6-8C5 (150 μg, i.p., n = 6) 24 hours before assessment of anti-GBM antibody-induced platelet recruitment. Data were compared with that of mice treated with anti-GBM antibody alone (n = 6). Data are shown as mean ± SEM. *P < 0.05 versus anti-GBM antibody-treated group for each time point.