Figure 8.

Platelet-derived P-selectin contributes to leukocyte recruitment in the inflamed glomerulus. Intravital microscopy was used to assess anti-GBM antibody-induced glomerular leukocyte recruitment in P-selectin chimeric mice. The following mice were examined: wild-type mice reconstituted with wild-type bone marrow (wild-type into wild-type, n = 6), P-selectin^−/− mice reconstituted with wild-type bone marrow (wild-type into P-sel^−/−, n = 7), wild-type mice reconstituted with P-selectin^−/− bone marrow (P-sel^−/− into wild-type, n = 7), and P-selectin^−/− mice reconstituted with P-selectin^−/− bone marrow (P-sel^−/− into P-sel^−/−, n = 6). Data show glomerular leukocyte adhesion assessed two hours after administration of anti-GBM antibody (20 mg, i.v.). Data are shown as mean ± SEM. *P < 0.05 versus wild-type into wild-type and wild-type into P-sel^−/−.