Figure 6.

Protease activity of HtrA2 is required for ITGA7 mediated cell deaths. A: Immunoblotting analysis of PITT1 cells transfected with wild-type or protease-null mutant HtrA2 (S306A), treated with or without a morpholino oligo specific for the leader sequence of HtrA2 (mH2, 125 pmol) or its scramble control (scr), and induced with or without tetracycline. The protein extracts were immunoblotted with the indicated antibodies. B: Representative FACS analysis of cell death of replicate samples from A, stained with fluorescein-conjugated Annexin V and propidium iodide, and analyzed in a LSC-II flow cytometer.