Figure 7.

IL5-induced eosinophilia leads to colon shortening, with CD34 expression on colon infiltrating eosinophils. Colon tissues from unchallenged, non-DSS-treated, IL5^Tg and Cd34^−/−IL5^Tg at steady state were excised, measured. and processed for flow cytometry. Blood, bone marrow and spleen tissues were also processed for flow cytometry as described in Materials & Methods. A: Baseline colon length of Cd34^+/+ and Cd34^−/− animals with or without the IL5 transgene. Histogram profiles of CD34 expression on total CD45⁺ cells (B) and CD45⁺/CCR3⁺SSC^hi cells (C) in peripheral blood, marrow, spleen, and colon isolates. For all histograms, black lines represent staining on cells from Cd34^+/+IL5^Tg tissues and gray-filled histograms represent background staining of cells from Cd34^−/−IL5^Tg tissues. (Histograms are representative of three separate experiments; n = 9 to 12 for colon length; **P < 0.01; Error bars = SEM).