Figure 5.

Restoration of oligodendrocyte/myelin status and oligodendrocyte numbers in CA1 region of the hippocampus in the brains of rAAV-scFvAβ_KDEL^IB injected 3×Tg-AD mice. Coronal sections from 6-month-old rAAV-scFvphe_KDEL^IB (A, B, E, F, I, J) or rAAV-scFvAβ_KDEL^IB (C, D, G, H, K, L) injected Non-Tg (A, C, E, G, I, K) and 3×Tg-AD mice (B, D, F, H, J, L) were stained with antibodies specific for CNPase (A–D), MBP (E–H), or neurofilament (I–L), and representative microscopic images are depicted (A–L). Insets represent ×2 magnification of the original selection and depict CNPase-positive cell body staining patterns in the hippocampus (A–D). Quantitation of CNPase (M), MBP (N), and NF (O) immunopositivity was performed by calculating integrated densities across each genotype and treatment group. Scale bars = 5 μm. Coronal sections from 6-month-old rAAV-scFvAβ_KDEL^IB or rAAV-scFvphe_KDEL^IB injected Non-Tg and 3×Tg-AD mice were also immunostained for the CC-1 and Olig2 oligodendrocyte markers. Representative images for CC-1 (P–S) and Olig2 (T–W) for the CA1 hippocampal region are depicted for the 6-month-old rAAV2 vector-injected Non-Tg (P, R, T, V) and 3×Tg-AD mice (Q, S, U, W). Quantitative analyses were also performed to assess the numbers of CC-1 (X) and Olig2 positive cells (Y) in the CA1 region of the hippocampus. Scale bars = 5 μm. Error bars indicate SD N = 6 per genotype per treatment. *P < 0.05; **P < 0.01.