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. 2010 Sep;177(3):1448–1458. doi: 10.2353/ajpath.2010.091081

Figure 3.

Figure 3

The expression of OPN in MMP-12+/+ and MMP-12−/− mice changes slightly with EAE. A: Expression of transcripts encoding OPN. Values are mean ± SEM of expression of transcript normalized to that of a house keeping gene, GAPDH. There are four C57BL/6 mice per control histogram and six C57BL/6 mice per EAE histogram. Samples were collected from mice at peak disease (clinical score of >8). There was no statistical significant difference in OPN transcripts between MMP-12−/− and MMP-12+/+ mice. B: A representative Western blot for OPN in crude extract of EAE-afflicted spinal cord at peak disease from a wild-type or MMP-12−/− mouse. C: Quantification of some of the bands (crude protein extract, top six panels; WGA extract, bottom two panels) detected by the anti-OPN antibody in B, across four mice per genotype and where spinal cord samples were obtained at peak disease. Although several bands were apparent in crude protein extracts, where only some were quantitated to highlight differences between genotypes, WGA extract of OPN showed only two dominant bands with the 58 kDa band changing significantly between groups. Values are mean ± SEM. *P < 0.05, t-test.