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. 2010 Sep;177(3):1470–1479. doi: 10.2353/ajpath.2010.091291

Figure 1.

Figure 1

A: Higher levels of PRDM1α mRNA in non-GCB-type DLBCL. Mean levels of PRDM1α transcripts in different groups of DLBCL (U266 = 1). Non-GCB DLBCL have higher PRDM1α mRNA expression compared with GCB DLBCL. B: Absence of PRDM1 promoter hypermethylation in GCB-type primary DLBCL and DLBCL cell lines. The CpG island identified in the PRDM1 promoter is depicted on the top of the figure (shaded). The approximate transcription start site is indicated by an arrow. A 361-bp sequence containing a total of 32 CpG sites within the CG island was amplified by a pair of primers that do not contain CpG sites from DNA extracted from GCB-type primary DLBCL (#18 to #25) and DLBCL cell lines (OCI-LY1, OCI-Ly7 and SUDHL-6). At least 10 clones were sequenced per sample, and the methylation status of each CpG site for each clone was indicated in the lollipop diagram. The open circle denotes an unmethylated CpG; the filled circle denotes a methylated CpG. None of the samples demonstrated promoter hypermethlyation in the PRDM1 gene.