Figure 6.

Let-7 down-regulated PRDM1 expression in U2932 DLBCL cell line. Control miRNA (NC), let-7a, and let-7b were transfected into U2932 cells by nucleofection. Nuclear extracts were prepared from each transfectant after 24 to 48 hours, and PRDM1α levels were measured by Western blot analysis using the anit-PRDM1 monoclonal antibody. Lamin B was used as a loading control. Normalized PRDM1α levels were expressed as a percentage relative to the negative control transfectants. The histogram was derived from two (let-7f) or four independent transfection experiments (let-7a and let-7b). Two representative Western blots are also shown.