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. 2010 Sep;177(3):1480–1490. doi: 10.2353/ajpath.2010.100052

Figure 6.

Figure 6

Analysis of IGSF4 promoter methylation in serum samples from HL patients and normal controls using nested MSP. A: IGSF4 methylation was detectable in the sera of 14/18 cases of HL. L591 was used as a positive control for MSP and USP. B: IGSF4 methylation was only detectable weakly in 1/17 normal sera. MSP products were confirmed by direct sequencing (see Supplemental Figure 3 at http://ajp.amjpathol.org). L591 was used as a positive control for MSP and USP. MSP results of ANKRD30A are shown here as a methylated positive and unmethylated negative control for normal sera. Human placenta was used as positive control for MSP and USP of ANKRD30A.