Table 4.
Correlation of IGSF4 Methylation with its Protein Expression in Primary HL
| HL case no. | IGSF4 expression in HRS cells by IHC | Methylation status (whole tumor/HRS cells) |
|---|---|---|
| SN6 | Normal | U |
| SN8* | Normal | Weak M/M |
| P3* | Moderate | U/U |
| SN1 | Absent/low | M |
| SN2 | Absent/low | M |
| SN3 | Absent/low | M |
| SN4 | Absent/low | M |
| SN5 | Absent/low | U |
| SN7 | Absent/low | M |
| P1* | Absent/low | Weak M/M |
| ad1* | N/A | M/M |
| P2* | N/A | U/U |
HRS cells were microdissected from some cases and subject to nested MSP. M, methylated IGSF4 detected; U, only unmethylated alleles detected; weak M, predominantly unmethylated alleles, but some low level methylation detected. MSP results of either whole tumor (taken from Table 2), or microdissected HRS cells from some cases (marked by an asterisk) (see Table2). N/A-paraffin sections not available for IHC.