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. 2010 Sep;177(3):1491–1502. doi: 10.2353/ajpath.2010.091021

Figure 1.

Figure 1

Hypoxia positively upregulates mRNA levels of lysyl oxidase (LOX), Vascular Endothelial Growth Factor (VEGF), Hypoxia Induced 2 (HIG2), and Prominin1 (CD133), and in some cells KLF4 and SOX2 in the established glioma neurosphere lines HSR-GBM2 and HSR-GBM1, and in freshly resected, GBM tumors, JHH-GBM10, JHHGBM11, and JHH-GBM12. All data was normalized to β-actin and is represented as relative expression (relative to normoxia; A). Twenty-four hours exposure to hypoxia induces HIF1α and CD133 protein expression in HSR-GBM1. GAPDH was used as a protein loading control (B). In HSR-GBM1, hypoxia positively upregulates mRNA levels of the Notch ligands, JAG1 and JAG2, as well as its targets, Hey2 and Hes1 (C). In HSR-GBM1, CD133-positive cells are two-fold more clonogenic in comparison with CD133-negative cells as indicated by a 2-fold increase in the development of large (>100mm) CSC containing spheres (**P < 0.01, two sided t-Test; D).