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. 2010 Sep;177(3):1491–1502. doi: 10.2353/ajpath.2010.091021

Figure 3.

Figure 3

A: Constitutive expression of oxygen-stable HIF1αP402A/P564A (left panel) increased levels of CD133 protein expression (right panel) in HSR-GBM1 C5 and E11 as compared with the vector control infected parent line (P). B: Flow cytometric analysis for CD133 of normoxic and hypoxic HSR-GBM1 C5 and E11 cell lines overexpressing HIF1αP402A P564A showed increased baseline levels of CD133 positive cells, which further increases following 24 hours of hypoxia. Unconjugated CD133 (pure) was used as threshold control. C: Side population was increased in HSR-GBM1 cultures that were exposed for 24 hour to hypoxia as compared with normoxia grown cells.