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. Author manuscript; available in PMC: 2011 Jul 30.
Published in final edited form as: Mol Cell. 2010 Jul 30;39(2):259–268. doi: 10.1016/j.molcel.2010.07.005

Figure 5. DNA binding activity of FAAP24 is required for ICL-induced RPA foci formation.

Figure 5

(A) A scheme showing full-length and C-terminal truncated FAAP24. (B) Immunoblot showing wild-type FAAP24 (WT) but not C-terminal truncated FAAP24 mutant (N150) rescued defective RPA2 phosphorylation in FAAP24-depleted HeLa cells. FAAP24 shRNA cells, transfected with empty-vector (EV), Myc-tagged WT or N150 FAAP24, were treated with 1 μM of MMC for 10 hr. Whole cell extracts (WCE) and chromatin enriched extracts (Chromatin) were analyzed by immunoblotting using indicated antibodies. (C) Quantification showing recovered RPA foci by WT but not N150 FAAP24. Cells as described in (B) were treated with MMC 1 μM for 6 hr. RPA foci were detected using anti-RPA2 antibody. The percentage of cells containing RPA foci was determined by counting at least 200 cells from each sample. Data are represented as mean ± SD from three independent experiments.