Figure 3.

Detection of BmK IT1 expression in Aedes albopictus larvae transduced with recombinant Aedes aegypti densovirus (AeDNV) mixed stock. (A) Reverse transcription-polymerase chain reaction (RT-PCR) techniques were performed on total RNA samples extracted from Ae. albopictus larvae. BmK IT1 messenger RNA (mRNA) accumulation in larvae at 24, 36, 48, 60, and 72 h post-transfection. (B) Western blot analysis for BmK IT1 expressed in larvae. Proteins were extracted at 24, 36, 48, 60, and 72 h; proteins were probed with anti-BmK IT1.