Figure 1.

The TIG1 Gene Replacement Construct and Mutants.

(A) Schematic diagram of the genomic region of the TIG1 and hph genes. Primers F1, R1, F2, R2, NF1, and NR1 were used to generate the TIG1 gene replacement constructs and mutant screens. P, PstI.

(B) DNA gel blots of PstI-digested genomic DNA were hybridized with a TIG1 fragment (left panel) and the hph gene (right panel) as the probes. Guy11 and 70-15 are the wild-type strains. KT-1, KT-2, and KT-4 are three independent tig1 null mutants.