Figure 4.

Penetration Assays with Rice Leaf Sheath Epidermal Cells.

(A) Extensive invasive hyphae were developed by Guy11 inside plant cells by 72 h after inoculation. The tig1 mutant (KT-1) had only limited growth of primary invasive hyphae.

(B) When stained with DAB, rice cells penetrated by the tig1 mutant accumulated ROS around fungal hyphae. No obvious ROS accumulation was observed in plant cells penetrated by Guy11. A, appressorium; PI, primary invasive hyphae; SI, secondary invasive hyphae.

Bars = 10 μm.