Figure 1.

Analysis of S. cerevisiae YAP1 leader sequence in the 5′ UTR of Photinus luciferase monocistronic mRNA. (A) Schematic representations of the monocistronic mRNAs −/P, YAP1/P, and CLN3/P are indicated. Yeast were transformed with the pMyr constructs that expressed these mRNAs, monocistronic mRNA expression was induced by galactose, and Photinus luciferase activities were determined 3 h after induction. Photinus luciferase activities were divided by the Photinus luciferase mRNA levels (shown in B). In this figure and those that follow, the ordinate for the histograms is formed by the constructs indicated immediately to the left. (B) Northern blot hybridizations of total RNA purified from cells transformed with the constructs indicated, and probed with a RNA probe complementary to the Photinus luciferase gene. The position of the 18S rRNA is indicated. Photinus luciferase mRNA levels were quantified by using a PhosphorImager. Similar results were obtained in three independent experiments.