Figure 3.

Analysis of S. cerevisiae p150-transcribed leader sequence in the 5′ UTR of CAT monocistronic mRNA. (A) Schematic representations of the monocistronic mRNAs are indicated, without (−/CAT, p150/CAT) or with (−/CATh, p150/CATh) a hairpin structure at the 5′ end of the mRNA. Yeast were transformed with the pGAD10 constructs that overexpressed these mRNAs. CAT activities were determined, divided by CAT mRNA levels, and represented on a logarithmic scale. (B) Northern blot hybridizations of total RNA purified from cells transformed with the constructs indicated and probed with an RNA probe complementary to the CAT gene. The position of the 18S rRNA is indicated. Similar results were obtained in two independent experiments.