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. 2010 Jul 2;22(7):2444–2458. doi: 10.1105/tpc.110.075358

Figure 2.

Figure 2.

Coexpression of ATR1 and RPP1 in Tobacco Triggers the HR.

(A) RPP1-WsB-HA induces HR in tobacco upon Agrobacterium-mediated coexpression with full-length ATR1-Emoy2-Citrine, ATR1δ15-Emoy2-Citrine, or ATRδ51-Emoy2-Citrine. All constructs were expressed under the control of the constitutive cauliflower mosaic virus 35S promoter. The photograph was taken 48 h post infiltration.

(B) Protein immunoblot probed with α-HA antibody showing relative levels of protein expression of RPP1-WsB-HA in tobacco driven by either its native promoter (NP) or cauliflower mosaic virus 35S promoter (35S). Ribulose-1,5-bis-phosphate carboxylase/oxygenase (Rubisco), stained on the blots with Ponceau S stain, is shown as a loading control.

(C) Protein immunoblot probed with α-GFP antibody showing relative levels of protein expression of the full-length (FL) ATR1-Emoy2-Citrine, ATR1δ15-Emoy2-Citrine, and ATRδ51-Emoy2-Citrine in tobacco at 24 h post infiltration. Rubisco is shown as a loading control.