Figure 7.

The TIR Domain Is Sufficient for Triggering the HR in Tobacco.

(A) The TIR truncation (amino acids 1–266) of RPP1-WsB fused to GFP is able to elicit effector-independent HR in tobacco.

(B) An A206K mutation in GFP that disrupts its ability to form dimers compromises the ability of the TIR to autoactivate.

(C) The E158A mutation in the TIR domain compromises its ability to trigger effector-independent HR.

(D) and (E) Protein immunoblots probed with α-GFP antibody showing relative expression levels of the GFP-tagged RPP1 constructs. Samples were taken at 48 h post infiltration. Rubisco is shown as a loading control. At 48 h post infiltration, HR triggers overall protein degradation (i.e., lower levels of Rubisco), while levels of TIR-GFP are unchanged.