Figure 3.

N-terminal Fragment of phyB Is Unstable in Transgenic Plants.

(A) phyB levels in wild-type (WT) Arabidopsis seedlings (Ler) under D and R for 24 h in the presence or absence of MG132.

(B) Protein immunoblot analysis of transgenic Arabidopsis seedlings (NGG-NLS/B and CG/B) under D and R for 24 h in the presence or absence of MG132. NGG-NLS and CG levels were detected by anti-GFP antibody. Tubulin (Tub) was detected as a loading control.

(C) Posttranslational decay experiment showing fast decay rate of NGG-NLS under R light. Blots were probed with anti-GFP antibody.