Figure 5.

pif Mutants Accumulate Higher phyB Levels Compared with the Wild Type.

(A) phyB decay is delayed in pif mutants and cop1-4 under R light.

(B) phyB levels in pif3 pif4, pif4 pif5, and cop1-4 compared with the wild type. Samples (0, 8, and 12 h after cycloheximide treatment) were chosen from (A) and analyzed on the same blot.

(C) to (E) Nuclear phyB levels are elevated in cop1-4 (C), pif3 pif4 (D), and pif4 pif5 (E).

(F) Direct comparison of nuclear phyB levels in the wild type (Col), pif4 pif5, and cop1-4.

In (C) to (F), sample fractionations and antibodies for marker proteins were identical to those of Figures 4A to 4C.