Figure 7.

COP1 Ubiquitinates Other Type II Phytochromes through Direct Interaction.

(A) In vitro pull-down assays showing interaction between N- or C-terminal regions of PhyB-E and COP1. Asterisks indicate the original size of N- or C-terminal regions of PhyB-E.

(B) COP1-mediated ubiquitination of N-terminal regions of PhyB-D or N- and C-terminal regions of PhyE. Asterisks indicate original size of the substrates. In vitro ubiquitination assay was done with E1 ubiquitin activating enzyme and E2 ubiquitin conjugating enzyme in the presence or absence of GST-COP1 (G-COP1).