Figure 4.

Expression Pattern of Hj WUS in H. japonicum Shoots.

(A) and (B) Longitudinal sections of an initiating leaf.

(A) Hj WUS was detectable within the first leaf primordium (L1) arising from the inner root tissue near the root margin.

(B) Negative control using Hj WUS sense probe.

(C) to (G) Longitudinal sections of reproductive shoots at successive stages.

(C) Hj WUS was expressed within the youngest and second youngest bract primordia (B1 and B2) in a young shoot without flowers.

(D) Hj WUS was detected in B1 and at the site where the floral meristem would arise (FM).

(E) Expression of Hj WUS was restricted to within the emerging FM, which is flanked by spathella primordia.

(F) Weak restricted expression of Hj WUS in the center of a carpel (C).

(G) Hj WUS expression was detected in nucelli (N).

Asterisks indicate vacuolated cells. B1 to B5, youngest to oldest bracts; C, carpel; FM, floral meristem; II, inner integument; L1, youngest leaf; N, nucellus; OI, outer integument; Pl, placenta; R, root; S, spathella; T, tepal. Bars = 50 μm.