Figure 4.

SEP1 Is Required for Appressorium-Mediated Plant Infection by M. oryzae.

(A) Bar charts to show the frequency of appressorium development in M. oryzae sep1^G849R temperature-sensitive mutants. Error bars are 1 se.

(B) Conidial suspensions of two independent M. oryzae sep1^G849R transformants were incubated in conditions to allow appressorium development, which was recorded after 24 h. Bars = 10 μm.

(C) M. oryzae sep1^G849R mutants were unable to cause rice blast disease. Leaves from the dwarf Indica rice (O. sativa) cultivar, CO-39, following inoculation with 5 × 10⁴ spores mL⁻¹ of a sep1^G849R mutant and Guy-11. Following inoculation, rice plants were incubated initially for 24 h at either the permissive temperature of 24°C or a semirestrictive temperature of 29°C. All plants were then transferred to 24°C and leaves harvested 5 d later.