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. Author manuscript; available in PMC: 2010 Aug 27.
Published in final edited form as: Glycoconj J. 2008 Sep 5;26(3):335–347. doi: 10.1007/s10719-008-9181-1

Fig. 4.

Fig. 4

SSEA-3 expression on embryos from control and T-syn mutant females. Late-stage blastocysts were included as negative controls (dashed arrows). A. Phase contrast of control embryos obtained from T-synF/F females mated to T-syn+/− males. B. Control embryos from A binding PNA. C. Control embryos from A binding the SSEA-3 MAb. Only the 4–8 cell embryos fluoresced brightly. D. Phase contrast of embryos obtained from T-synF/F:ZP3Cre mutant females mated with T-syn+/− males. E. The T-syn+/− and T-syn−/− embryos from D binding PNA. PNA-negative T-syn−/− embryos are circled. F. The same T-syn+/− and T-syn−/−embryos from D binding the SSEA-3 MAb. PNA-negative SSEA-3-positive T-syn−/− embryos are circled. A′-F′. The area marked by each dotted square in A-F is magnified. Images are at the same magnification and the same exposure. Solid arrows indicate PNA-negative mutant embryos. The images are representative of 3 independent experiments with 2 eggs and 21 4–8 cell embryos, half of the total generated by 6 control females, 3 eggs and 21 4–8 cell embryos, half of the total generated by 6 T-syn mutant females.