Fig. 2.

Rs1 protein in the presence of Ca²⁺ reorganizes stacks of multiple bilayers in favor of a single, expanded, 2D bilayer. A. The top left image shows stacked bilayer patches in equilibrium before Rs1 injection. Up to five-level stacked patches are present. Rs1 was injected after image A was acquired. B–F. The same 3×3 μm² region was imaged continuously after Rs1 injection. Notice the gradual depletion of overlying patches and the simultaneous expansion of the basal bilayer. Also noticeable is the progressive reorganization of the phase configuration on the basal bilayer.