| water: | to 200 μl | |
| 5× transcription buffer: | 40 μl | 1× |
| 100 mM DTT: | 10 μl | 5 mM |
| 25 mM rNTP (ROCHE): | 30 μl | 3.75 mM |
| template: | 50 mg/ml | |
| Pyrophosphatase: | 1 U/ml | |
| T7 RNA polymerase: | 1000–2000 U/ml |
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