Table 1.

Three-Day Assay Signal Window and Z-Factor Determination

Class	Day	Plate	Max/Min	Mean	SD	CV	Z-Factor	SB
Intraplate	1	1	Max	1,436.18	75.02	5.22	0.56	5.92
		2	Max	1,356.41	94.46	6.96	0.52	5.48
		3	Min	242.78	98.59	40.61	0.48	5.59
		4	Min	262.21	111.29	42.44	0.44	5.17
		All plates					0.48	5.53
	2	1	Max	1,149.79	64.67	5.62	0.59	5.82
		2	Max	1,184.73	64.96	5.48	0.57	5.83
		3	Min	197.44	65.81	33.33	0.60	6.00
		4	Min	197.29	71.5	36.24	0.59	6.01
		All plates					0.58	5.91
	3	1	Max	879.71	59.28	6.74	0.64	7.56
		2	Max	866.21	58.34	6.74	0.63	7.34
		3	Min	116.34	33.39	28.7	0.63	7.45
		4	Min	119.91	34.66	28.91	0.63	7.22
		All plates					0.63	7.39
Interplate	1	1 and 2	Max	1,396.3	94.15	6.74	0.48	5.53
		3 and 4	Min	252.49	105.56	41.81
	2	1 and 2	Max	1,167.26	62.17	5.75	0.58	5.91
		3 and 4	Min	197.37	68.7	34.81
	3	1 and 2	Max	872.96	59.19	6.78	0.63	7.39
		3 and 4	Min	118.12	34.07	28.84
Day to day	1 and 2	All plates	Max	1,281.78	140.71	10.98	0.34	5.70
			Min	224.93	93.22	41.44
	2 and 3	All plates	Max	1,020.11	160.19	15.7	0.21	6.47
			Min	157.75	67.15	42.57

The p53-hDM2 protein–protein interaction biosensor data presented in this table were generated in 3 independent experiments of 2 full plates each of the maximum (0.5% dimethyl sulfoxide) and minimum (10 μM Nutlin-3) plate controls conducted on 3 separate days.