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. Author manuscript; available in PMC: 2010 Aug 27.
Published in final edited form as: Mol Cancer Res. 2008 Jan;6(1):119–126. doi: 10.1158/1541-7786.MCR-07-0090

FIGURE 3.

FIGURE 3

IL-1α promoter activity is enhanced and IL-1α protein expression is increased following suppression of Osx. A. The IL-1α promoter reporter construct was transfected into different cells as indicated. Luciferase activity was measured 24 h later. Relative fold activity was calculated as the normalized luciferase value divided by the normalized luciferase value in Dunn or DLM8 cells. B. IL-1α protein in Dunn, Dunn-si-c, Dunn-si-2, DLM8, DLM8-si-c, and DLM8-si-1 cell lysate was quantified by ELISA. Relative fold was calculated as the normalized protein concentration divided by the normalized protein concentration in Dunn or DLM8 cells.