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. Author manuscript; available in PMC: 2010 Aug 27.
Published in final edited form as: Mol Cancer Res. 2008 Jan;6(1):119–126. doi: 10.1158/1541-7786.MCR-07-0090

FIGURE 4.

FIGURE 4

Mutating one Sp1-binding site on IL-1α promoter increased its activity. IL-1α promoter activity was increased after mutating the Sp1-binding site on the IL-1α promoter in Dunn and DLM8 cells. Wild-type, mutant, or irrelevant mutant promoter reporters were transfected into Dunn and DLM8 cells as indicated. After 24 h, luciferase activity was measured. Relative fold activity was calculated as the normalized luciferase value divided by the normalized luciferase value of wild-type promoter reporter.