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. 2010 Aug 27;5(8):e12458. doi: 10.1371/journal.pone.0012458

Figure 2. shRNA-mediated downregulation of uPAR and uPA increases HMEC apoptosis and reduces U87, U87 SPARC and HMEC proliferation.

Figure 2

(A) U87, (C) U87 SPARC and (E) HMEC were transfected with pSV, puPA, puPAR or pU2, and the cells were trypsinized followed by PI staining as per standard protocols. 10,000 cells were sorted by flow cytometer to determine the DNA content of the cells. (B), (D) and (F) Briefly, 1.5×105 U87, U87 SPARC and HMEC were transfected with pSV, puPAR, puPA or pU2 in vitronectin-coated 96-well microplates under serum-free conditions. The number of viable cells was assessed by MTT assay. *p<0.05, **p<0.01. (G) Graph represents the percentage of apoptosis in cells before and after transfection with shRNA. Data shown are the mean ± SD values from four different experiments. **p<0.01.