Figure 3. shRNA against uPA and uPAR downregulates secreted pro-angiogenic factors.

HMEC and cancer cell co-cultures were incubated with Ray Biotech angiogenesis antibody array 1 and 2 as per the manufacturer's instructions (Fig. A & C). Results are represented from three independent experiments. Highlighted proteins are angiogenin, epidermal growth factor (EGF), GRO, IL-6 (interleukin 6), GM CSF (granulocyte macrophage colony stimulating factor), and Ang-1 (angiopoeitin-1). (B) and (D) Graphs represent quantification of antibody array using NIH ImageJ software. *p<0.05, **p<0.01.