Figure 2.

Expression and purification of wild-type (wt) rat PAH and mutants shown on SDS/PAGE gels (10% Bis-Tris gels run with Mops running buffer, NOVEX, San Diego). Gels were stained with Colloidal blue staining kit (NOVEX). (A) Lanes 1 and 4, crude E. coli extracts; lanes 2 and 5, flow-through from pterin column after applying crude extract; lanes 3 and 6, the eluate with NaHCO₃/NaOH (pH 10.8); lane 7, protein-standard markers (Mark 12, NOVEX). (B) Purified wt and mutants. Names are shown on top of the lanes. M12 is the marker.