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. 2001 Feb 6;98(4):1537–1542. doi: 10.1073/pnas.031561698

Table 1.

Oligonucleotide primers used in this paper

Name Sequence Size Notes
o464 TGGCATATGGCAGCTGTTGTATTG 24mer 5′ primer for rat PAH
o465 TGGCATATGGAATCCAGACCTTCTC 25mer 5′ primer for human PAH
N-rev TTAGACAGTGGCACCAATGTCAT 23mer Antisense primer down-stream from Bsu36I site
N-15 TGGCATATGTCAGACTTTGGGCAGGAAA 28mer Introduction of initiator ATG upstream of residue 16
N-26 TGGCATATGGACAACTCCAATCAAAATG 28mer Introduction of initiator ATG upstream of residue 27
N-40 TGGCATATGCTCAAAGAGGAAGTTGGTG 28mer Introduction of initiator ATG upstream of residue 41
N-59 TGGCATATGATCAACCTGACACACATTG 28mer Introduction of initiator ATG upstream of residue 60
o265 TAATACGACTCACTATAGGG 20mer Sense primer upstream from XbaI and NdeI sites
o560 GACAACTGCAATCAAAATGGTGC 23mer 29Cys(+)
o561 TTGATTGCAGTTGTCTTCAATATAGC 26mer 29Cys(−)
o562 GACAACTCCAATCAAAATGGTGC 23mer 29Ser(+)
o563 TTGATTGGAGTTGTCTTCAATATAGC 26mer 29Ser(−)
o569 TGAAGACAACGCCAATCAAAATGGTG 26mer 29Ala(+)
o570 TGAAGACAACATGAATCAAAATGGTG 26mer 29Met(+)
o571 TGAAGACAACCTCAATCAAAATGGTG 26mer 29Leu(+)
o572 TGAAGACAACGACAATCAAAATGGTG 26mer 29Asp(+)
o573 TGAAGACAACAACAATCAAAATGGTG 26mer 29Asn(+)
o574 TGAAGACAACCGCAATCAAAATGGTG 26mer 29Arg(+)
o575 GTTGTCTTCAATATAGCTTGT 21mer Antisense primer upstream from site serine-29