o464 |
TGGCATATGGCAGCTGTTGTATTG |
24mer |
5′
primer for rat PAH |
o465 |
TGGCATATGGAATCCAGACCTTCTC |
25mer |
5′ primer
for human PAH |
N-rev |
TTAGACAGTGGCACCAATGTCAT |
23mer |
Antisense primer
down-stream from Bsu36I site
|
N-15 |
TGGCATATGTCAGACTTTGGGCAGGAAA |
28mer |
Introduction
of initiator ATG upstream of residue 16
|
N-26 |
TGGCATATGGACAACTCCAATCAAAATG |
28mer |
Introduction
of initiator ATG upstream of residue 27
|
N-40 |
TGGCATATGCTCAAAGAGGAAGTTGGTG |
28mer |
Introduction
of initiator ATG upstream of residue 41
|
N-59 |
TGGCATATGATCAACCTGACACACATTG |
28mer |
Introduction
of initiator ATG upstream of residue 60
|
o265 |
TAATACGACTCACTATAGGG |
20mer |
Sense primer upstream from
XbaI and NdeI sites
|
o560 |
GACAACTGCAATCAAAATGGTGC |
23mer |
29Cys(+)
|
o561 |
TTGATTGCAGTTGTCTTCAATATAGC |
26mer |
29Cys(−)
|
o562 |
GACAACTCCAATCAAAATGGTGC |
23mer |
29Ser(+)
|
o563 |
TTGATTGGAGTTGTCTTCAATATAGC |
26mer |
29Ser(−)
|
o569 |
TGAAGACAACGCCAATCAAAATGGTG |
26mer |
29Ala(+)
|
o570 |
TGAAGACAACATGAATCAAAATGGTG |
26mer |
29Met(+)
|
o571 |
TGAAGACAACCTCAATCAAAATGGTG |
26mer |
29Leu(+)
|
o572 |
TGAAGACAACGACAATCAAAATGGTG |
26mer |
29Asp(+)
|
o573 |
TGAAGACAACAACAATCAAAATGGTG |
26mer |
29Asn(+)
|
o574 |
TGAAGACAACCGCAATCAAAATGGTG |
26mer |
29Arg(+)
|
o575 |
GTTGTCTTCAATATAGCTTGT |
21mer |
Antisense primer upstream
from site serine-29 |