Table 1. Predicted expression of the of lux genes before and after codon-optimization.

Gene	Predicted Start Position	Length	% GC	Number of Nucleotide Substitutions	Probability of Recognition as an Exon	Exon Score
wtluxC	1	1443	37%	N/A	0.921	50.78
coluxC	1	1443	60%	449	0.999	360.72
wtluxD	1	924	38%	N/A	0.875	29.53
coluxD	1	924	59%	294	0.999	238.19
wtluxE	102	1087	38%	N/A	0.443	33.11
coluxE	1	1113	60%	331	0.999	271.01
wtfrp	1	613	47%	N/A	0.715	30.70
cofrp	1	723	64%	249	0.999	179.43

GENSCAN translation prediction scores for expression of wild-type (wt) and codon-optimized (co) lux genes in a mammalian host cell (http://genes.mit.edu). Predicted start position is the predicted nucleotide sequence location for initiation based on the transcription of the nucleotide gene sequence. The predicted truncation in length of the wild-type luxE gene was due to the presence of a non-mammalian preferred start codon in the original gene sequence. Exon score interpretation: 0–50 weak, 50–100 moderate, >100 strong. Additional length in the codon-optimized version of the frp gene is the result of additional nucleotides added to introduce new restriction enzyme sites for improved cloning efficiency. N/A - not applicable.