. 2010 Aug 27;5(8):e12441. doi: 10.1371/journal.pone.0012441

Table 2. Bioluminescent production from unsupplemented HEK293 cells expressing P. luminescens lux genes.

Cell Line	Bioluminescent Detection (RLU/sec)
Cell Free Media	745 (±63)
Untransfected HEK293 Cells	655 (±44)
HEK293+pLux_AB+pLux_CDEfrp:WT	884 (±44)
HEK293+pLux_AB+pLux_CDEfrp:CO	7600 (±1241)

Actively growing HEK293 cells expressing various combinations of P. luminescens lux genes were harvested from culture and directly assayed for in vivo bioluminescent production. Lower levels of bioluminescent output from untransfected cells as opposed to cell free medium are most likely the result of background light absorption from endogenous chromophoric material in the cells. All values are the average of at least three trials and are reported with the standard error of the mean.