Figure 5. Bound DARPin 57.2 is not retained long term on macaque cells.

PBMCs were collected 30 minutes after DARPin injection and cultured in vitro for 7 days. Cells were harvested at the indicated timepoints, labeled for His and CD4 and analyzed by flow cytometry. (A) The MFIs (mean±SEM) of His staining on CD4⁺ cells are shown for animals injected with 300µg/kg DARPin 57.2 (n = 4) or DARPin E3_5 (n = 3). Baseline samples were taking from the same animals immediately before the injections (pre). The percentage of CD4⁺ cells (B) and the MFIs of CD4 expression (C) for the samples described in panel A were also measured over time.