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. 2010 Aug 27;5(8):e12445. doi: 10.1371/journal.pone.0012445

Figure 6. Lin28B-mediated repression of let-7 was partially responsible for regulating self-renewal in PC3 PDGF-D cells.

Figure 6

(A) The levels for let-7 family in PC3 Neo and PC3 PDGF-D cells were determined by using real time RT-PCR. (B) Real time RT-PCR was used to quantify the expression of let-7 in PC3 PDGF-D cells transfected with Lin28B siRNA for 3 days. (C) Photomicrographs showing prostaspheres generated from PC3 PDGF-D cells transfected with pre-let-7 or combination of pre-let-7 and Lin28B siRNA 14 days after transfection (bar:100 µm). (D) The cells were collected for prostasphere-forming assay after 14-day transfection. Let-7a, let-7b, let-7d and combination of Let-7a, let-7b, let-7d as well as co-transfection with let-7 and Lin28B siRNA decreased the number of prostaspheres. (E) The results from Western blot showed the expression of Lin28B, Sox2, Nanog, and Oct4 in PC3 PDGF-D cells transfected with pre-let-7 or co-transfected with pre-let-7 and Lin28B siRNA after 9-day transfection. *, p<0.05 compared to control; **, p<0.01 compared to control. (Con: control, a: pre-let-7a, abd: combination of pre-let-7a, pre-let-7a, pre-let-7d, al: combination of pre-let-7a and Lin28B siRNA, Lin28B-si: Lin28B siRNA, Neo: PC3 Neo cells, PDGF-D: PC3 PDGF-D cells).