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. 2010 Aug 10;10:85. doi: 10.1186/1471-213X-10-85

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Copyright ©2010 Liu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The production of erythroid cells from treated hEBs. (A)Amplification of erythroid cells in liquid culture.50000 hEB cells from co-culture could yield about 5000,000 erythroid cells in liquid medium.(B) Analysis of erythroid differentiation of treated hEBs by flow cytometry. Erythroid cells derived from treated hEBs were seeded in liquid medium and tested for expression of CD71 (the marker of early erythroid), GLA (the marker of matuered erythroid) cells by flow cytometry at different inducing times. (C)Statistical analysis for the cell surface antigen expression of hEBs-derived erythroid cells in liquid medium. The data represent the mean ± SEM from three experiments. *, P < 0.05 when compared with the average expression of CD71 or GLA at day 4.