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. 2010 Aug 12;8:43. doi: 10.1186/1477-5956-8-43

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Copyright ©2010 Villar et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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mRNA levels of genes coding for selected differentially expressed proteins. The genes encoding for differentially expressed unknown larval and guanine nucleotide-binding proteins were selected for mRNA analysis by real-time RT-PCR. mRNA levels were characterized in individual whole ticks naturally-infected with different pathogens using sequence-specific oligonucleotide primers. In all cases, matching groups of uninfected tick samples were analyzed concurrently for comparison. The graph depicts the infected to uninfected mRNA ratio (+SD) calculated by dividing normalized mRNA levels in infected ticks by the average of the normalized mRNA level in uninfected control ticks. Normalized mRNA levels were compared between infected and uninfected ticks by Student's t-Test (*P < 0.05). Abbreviations: R.s/E.c, R. sanguineus/E. canis; R.s/R.c, R. sanguineus/R. conorii; R.b/T.a, R. bursa/T. annulata; R.t/A.o, R. turanicus/A. ovis.