Table 3.
Rhipicephalus spp. tick differentially expressed proteins identified by MALDI-TOF MS and LC-MS/MS after saturation 2-D DIGE analysis.
| Spot numbera) |
Accession numberb) | Protein IDb) | Mw/pIc) | Protein Score d) | Number of matched peptides |
Sequence coverage (%) | Average -fold change e) | Tick/Pathogen |
|---|---|---|---|---|---|---|---|---|
| 1 | 3319897 | Albumin (Canis familiaris) | 67.8/5.3 | 123 | 11 | 19.0 | + 5.7 | R. sanguineus/E. canis |
| + 7.8 | R. bursa/T. annulata | |||||||
| 2 | 3319897 | Albumin (Canis familiaris) | 67.8/5.3 | 127 | 11 | 29.6 | +24.6 | R. sanguineus/E. canis |
| +17.5 | R. bursa/T. annulata | |||||||
| 3 | 3319897 | Albumin (Canis familiaris) | 67.8/5.3 | 125 | 13 | 30.1 | +38.8 | R. sanguineus/E. canis |
| +16.9 | R. bursa/T. annulata | |||||||
| 4 | 3319897 | Albumin (Canis familiaris) | 67.8/5.3 | 85 | 9 | 22.2 | +20.2 | R. sanguineusy/E. canis |
| +18.0 | R. bursa/T. annulata | |||||||
| 5 | 3319897 | Albumin (Canis familiaris) | 67.8/5.3 | 106 | 12 | 26.5 | +12.8 | R. sanguineus/E. canis |
| +26.9 | R. bursa/T. annulata | |||||||
| 6 | 3319897 | Albumin (Canis familiaris) | 67.8/5.3 | 128 | 11 | 28.0 | + 9.8 | R. sanguineus/E. canis |
| +28.6 | R. bursa/T. annulata | |||||||
| 7 | - | Not identified | - | - | - | - | -5.1 | R. bursa/T. annulata |
| -5.0 | R. turanicus/A. ovis | |||||||
| 8 | 5164373 | Pre-pro serum albumin (Ovis aries) | 69.2/5.8 | IT | 41 | 31.3 | -5.5 | R. bursa/T. annulata |
| -5.3 | R. turanicus/A. ovis | |||||||
| 9 | 193085052 | Albumin precursor (Capra hircus) | 66.3/5.6 | 88 | 17 | 27.6 | -5.2 | R. bursa/T. annulata |
| -5.1 | R. turanicus/A. ovis | |||||||
| 10 | 241157545 | Actin, putative (Ixodes scapularis) | 37.6/5.4 | 121 | 8 | 31.3 | -7.9 | R. sanguineus/R. conorii |
| -5.6 | R. bursa/T. annulata | |||||||
| -5.1 | R. turanicus/A. ovis | |||||||
| 11 | 59894747 | Actin (Ixodes ricinus) | 41.5/5.6 | 121 | 11 | 42.0 | - 5.5 | R. sanguineus/R. conorii |
| +6.6 | R. sanguineus/E. canis | |||||||
| - 5.9 | R. bursa/T. annulata | |||||||
| 12 | - | Not identified | - | - | - | - | - 5.1 | R. sanguineus/R. conorii |
| - 6.3 | R. bursa/T. annulata | |||||||
| - 5.7 | R. turanicus/A. ovis | |||||||
| 13 | 258499 | Haptoglobin heavy chain, HpH chain (Canis familiaris) | 27.3/5.8 | 104 | 7 | 37.1 | +36.7 +28.3 +12.4 |
R. sanguineus/R. conorii R. sanguineus/E. canis R. bursa/T. annulata |
| 14 | 215497327 | Enolase (Ixodes scapularis) | 21.5/8.9 | IT | 4 | 18.1 | - 8.2 | R. sanguineus/R. conorii |
| - 6.7 | R. bursa/T. annulata | |||||||
| - 5.5 | R. turanicus/A. ovis | |||||||
| 15 | 67083997 | Guanine nucleotide-binding protein (Ixodes scapularis) | 36.0/7.1 | IT | 2 | 9.4 | -28.7 - 7.9 - 5.1 |
R. sanguineus/R. conorii R. bursa/T. annulata R. turanicus/A. ovis |
| 16 | 157399341 | Unknown larval protein (Rhipicephalus annulatus) | 19.1/6.16 | IT | 3 | 17.5 | -25.6 -7.3 +18.5 |
R. sanguineus/R. conorii R. bursa/T. annulata R. turanicus/A. ovis |
| 17 | 157399341 | Unknown larval protein (Rhipicephalus annulatus) | 19.1/6.16 | IT | 5 | 11.1 | - 12.4 +17.8 |
R. bursa/T. annulata R. turanicus/A. ovis |
| 18 | 44887976 | Full hemoglobin subunit beta (Chrysocyon brachyurus) | 16.1/8.0 | 112 | 10 | 59.6 | +38.6 | R. sanguineus/R. conorii |
| + 9.5 | R. sanguineus/E. canis | |||||||
| +17.0 | R. bursa/T. annulata | |||||||
| 19 (mix) | 116618139 | Conjugal transfer protein, ATPase (Leuconostoc mesenteroides) | 95.8/5.8 | 86 | 16 | 17.1 | +62.4 | R. bursa/T. annulata |
| 44887976 | Full hemoglobin subunit beta (Chrysocyon brachyurus) | 16.1/8.0 | 75 | 8 | 56.2 | +13.1 | R. sanguineus/E. canis | |
| +38.6 | R. sanguineus/R. conorii | |||||||
| 20 | 44888810 | Full hemoglobin subunit alpha (Canis familiaris) | 15.3/8.0 | 111 | 7 | 63.8 | +66.7 + 7.0 +35.9 |
R. sanguineus/R. conorii R. sanguineus/E. canis R. bursa/T. annulata |
| 21 | 44888810 | Full hemoglobin subunit alpha (Canis familiaris) | 15.3/8.0 | IT | 7 | 29.3 | +41.5 | R. sanguineus/R. conorii |
| +30.7 | R. bursa/T. annulata | |||||||
a) Spot numbers refer to the 2 D gel proteins of interest that were analyzed by mass spectrometry (Figure 3).
b) Accession number and protein identity are listed according to the NCBInr database for the best match.
c) Abbreviations: Mw, molecular weight (kDa); pI, isoelectrical point.
d) Protein score is-10*Log(P), where P is the probability that the observed match is a random event, it is based on NCBInr database using MASCOT Peptide Mass Fingerprinting searching program. When IT appears, it means that these proteins were identified by LC-MS/MS.
e) + indicates an increase in protein expression and - indicates a decrease in protein expression in infected ticks.