Table 4.
Comparative analysis between protein and mRNA levels for selected differentially expressed tick proteins.
| Infected/Uninfected | |||
|---|---|---|---|
| Tick protein | Tick/pathogen | Protein | mRNA |
| Unknown larval protein | |||
| R. sanguineus/E. canis | Down | Up | |
| R. sanguineus/R. conorii | Unchanged | Unchanged | |
| R. bursa/T. annulata | Down | Unchanged | |
| R. turanicus/A. ovis | Up | Down | |
| Guanine nucleotide-binding protein | |||
| R. sanguineus/R. conorii | Down | Unchanged | |
| R. sanguineus/E. canis | Unchanged | Unchanged | |
| R. bursa/T. annulata | Down | Down | |
| R. turanicus/A. ovis | Down | Down | |
The mRNA levels of the differentially expressed tick guanine nucleotide-binding and unknown larval proteins were analyzed by real-time RT-PCR with RNAs from individual infected and uninfected ticks and correlated with protein levels determined by 2-D DIGE.